Product Code: ICAL07_N102
Glucose Biosensor on Cellulose Microfibers Through Layer-By-Layer Nanoassembly (Invited Paper - 40 Minute Presentation)
Kody Varahramyan, Louisiana Tech University; Ruston LA USA
Mangilal Agarwal, Louisiana Tech University; Ruston LA USA
Q Xing, Louisiana Tech University; Ruston LA USA
Yuri Lvov, Louisiana Tech University; Ruston LA USA
Presented at ICALEO 2007
A paper-based glucose biosensor on cellulose wood microfiber has been developed based on the layer-by-layer (LbL) nanoassembly technique. LbL nanoassembly is a versatile and easy to use technique based on sequential deposition of oppositely charged polyelectrolytes or nanoparticles on surfaces of different shapes and sizes. It is emerging as a key nanomanufacturing approach, allowing the realization of novel nanometer-scale multi-layered materials and structures, designed to have desirable characteristics and properties (e.g. chemical, mechanical, and electrical). Using LbL nanoassembly, a nanocomposite, consisting of conducting polymer poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT-PSS) and glucose oxidase (GOx), was coated to modify the surface of wood microfibers for paper-based glucose sensing application. The nanocomposite coated glucose biosensors on wood microfibers were thus used to make paper strips for glucose sensing. The results obtained show that nanocomposite layers of PEDOT-PSS and GOx display better response (35% change in conductivity of the coated nanocomposite film) in the presence of glucose as compared to the sensors fabricated using separate layer of PEDOT-PSS and GOx (20% change in conductivity of PEDOT-PSS film). The results have also been confirmed by fabricating similar sensors on glass substrate. Unlike commercially available glucose biosensors that are fabricated on rigid substrates and use costly metal electrodes such as platinum, the paper-based glucose biosensors under development in this work are flexible, use polymer electrodes, and are cost effective.
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