Product Code: JLA_24_4_042012

Authors:
Philipp Nachev
Funktionale und Interaktive Polymere, Institut fu¨r Makromolekulare und Technische Chemie, RWTH Aachen University, Forckenbeckstr. 50, D-52074 Aachen, Germany

Danielle D. van ’T Zand
Technical Chemistry I and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, D-45141 Essen, Germany

Vincent Coger
Department of Plastic, Hand- and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

Philipp Wagener
Technical Chemistry I and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, D-45141 Essen, Germany

Kerstin Reimers
Peter M. Vogt
Department of Plastic, Hand- and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

Stephan Barcikowski
Technical Chemistry I and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, D-45141 Essen, Germany

Andrij Pich
Funktionale und Interaktive Polymere, Institut fu¨r Makromolekulare und Technische Chemie, RWTH Aachen University, Forckenbeckstr. 50, D-52074 Aachen, Germany

Loading microgels with bioactive nanoparticles (NPs) often requires multiple synthesis and purification steps, and organic solvents or precursors that are difficult to remove from the gel. Hence, a fast and aqueous synthesis procedure would facilitate the synthesis of inorganic–organic hybrid microgels. Two microgel compounds were hybridized with laser-generated zinc oxide (ZnO) NPs prepared in a single-step procedure. ZnO NPs were formed by laser ablation in liquid, while the polymer microgels were synthesized in-situ inside the ablation chamber. Further, the authors report the preparation of two different microgel systems. The first one was produced without the use of chemical initiator forming hydrogels with ZnO NPs and diffuse morpholgy. Typical microgel colloids were also synthesized via a conventional chemical method in a preheated reaction chamber. The existence of microgel colloids partially loaded with ZnO NPs was confirmed in a transmission electron microscopy investigation. Fourier transform infrared spectroscopic measurements and dynamic light scattering verify the formation of polymer colloids. These initial results indicate the application potential of laser ablation in microgel precursor solution for the fabrication of polymeric carriers for inorganic nanoparticles. Preliminary biological tests using zinc chloride demonstrated negative dose effects on primary cell culture with zinc concentrations above 200 μM but no noticeable influence at 100 μM.