Product Code: ICA10_N104

Direct-Write Femtosecond Laser Ablation and DNA Combing and Imprinting for Fabrication of a Nanofluidic Device on Ethylene Glycol Dimethacrylate Polymer
Authors:
Yong Chae Lim, The Ohio State University; Columbus OH
Pouyan Boukany, Ohio State University; Columbus OH USA
Dave Farson, The Ohio State University; Columbus OH USA
Lee J. Lee, Ohio State University; Columbus OH USA
Presented at ICALEO 2010

Ordered nanochannel arrays are useful to manipulate liquids, ions and biomolecules with extremely high levels of spatial confinement for molecular separation and DNA/gene delivery applications. Using a novel DNA Combing and Imprinting (DCI) technique, well ordered arrays of microwells and nanoscale channels with size of order of 10nm created. Direct-write femtosecond laser ablation was employed to create micro-scale channels to connect inlet and outlet ports to the arrays to allow the system to be filled with desired reagents, genetic materials and cells. Single pulse and multiple pulse overlapped ablation tests were conducted on ethylene glycol dimethacrylate (EGMDA) polymer with a 50X objective. Atomic force microscope and scanning electron microscopy were employed to measure ablated width and depth. Single pulse ablation threshold fluence and incubation coefficient were found and were used to predict microchannel width. Femtosecond laser ablation was applied on the pre-patterned microwell/nanochannel array and fluorescent flow testing was performed to verify connectivity between the laser ablated microchannels and the DCI array nanochannels.