Product Code: ICAL06_M101

A Microfluidic Bioarray System using Laser-machined Surface Microstructures (Invited Paper)
Authors:
Tadatake Sato, National Institute of Advanced Industrial Science and Technology (AIST); Tsukuba, Ibaraki Japan
Aiko Narazaki, National Institute of Advanced Industrial Science and Technology (AIST); Tsukuba, Ibaraki Japan
Ryozo Kurosaki, National Institute of Advanced Industrial Science and Technology (AIST); Tsukuba, Ibaraki Japan
Thomas Gumpenberger, National Institute of Advanced Industrial Science and Technology (AIST); Tsukuba, Ibaraki Japan
Hiroyuki Niino, National Institute of Advanced Industrial Science and Technology (AIST); Tsukuba, Ibaraki Japan
Yoshizo Kawaguchi, National Institute of Advanced Industrial Science and Technology (AIST); Tsukuba, Ibaraki Japan
Presented at ICALEO 2006

Fabrication of microfluidic devices used for various bioanalysis often requires the high precision surface micro-structuring of the transparent materials. However, the transparent materials are generally hard and brittle, which makes precision surface micro-structuring difficult. As a solution, we have developed a technique for laser micromachining of transparent materials using organic solution, named laser-induced backside wet etching (LIBWE). It was reported that color-encoded microbeads with surface functional groups randomly arranged in a microwell array can be used as a microarray system. This concept of bead array is considered to meet a remaining challenge in microarray analysis systems, a cost-effective production of the device. Another challenge in microarray is a reduction in the reaction time. By designing microstructure appropriately the beads array can be formed within microchannels. Such device should meet both challenges. In the present work, we will report on a novel microfluidic device incorporating two-dimensional array of microbeads with 10 μm diameter. This work was in part supported by the Industrial Technology and Research Grant Program from NEDO of Japan.